Cytometry and Antibody Technology

We will be having a demo of a 200mW 561nm solid state laser on our MoFlo December 2nd and 3rd, 2008. Beckman Coulter will be in to install the laser as well as a detector block so we can assess the utility of such a laser for our sorting applications. The major benefit of this laser will be the ability to excite fluorochromes not normally excited at 488nm or 633nm, such as mCherry, RFP variants, and more. Other benefits of interest will be the stronger excitation of PE and PE-Tandems as well as the lack of spillover of blue excited fluorochromes like FITC and PerCPCy5.5 into PE channels. This will effectively increase the resolution of dimly stained populations, especially when identifying cells which are double positive for FITC and PE. We have a few scheduled demos of the laser with users of the facility, so if you’d like to see the laser in action, give us a call to find out what time we’ll be running samples on the 2nd and 3rd. Or, just drop by on either day to take a look at the setup.