After the post below regarding the Alexa Dye conjugations, I had a few questions from users, not about the Alexa Dyes available, but about the titration, and choosing the optimal antibody concentration. Titrating your antibodies will go a long way towards achieving...

The Fitch Monoclonal Antibody Facility (MAF) on campus is now offering antibodies coupled to many of the Alexa dye flavors from Invitrogen. As you may know, the Alexa Dyes are small molecular weight fluorochromes capable of producing bright fluorescence and minimal...