For some reason, it seems like the idea of compensation gets so much 'publicity'.  Everyone is always talking about compensation and how difficult it is.  New users of flow cytometry tend to think of this idea as something so complex that they end up stumbling on...

I'm not going to discuss the merits of transforming your log-scaled fluorescence flow cytometry data;  I'll leave that to the professionals.  What I will try to elucidate here is how I tweak the transformation using FlowJo (Mac version 9.3, sorry Windows people)....

For anyone doing sorting applications, I'd like to clear up a common misconception.  Just for the record, cell sorters are not sterile.  The instrumentation is not housed in a biosafety cabinet or hood, and therefore it is not possible to sort in a STERILE...