Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
When I was a graduate student, my PI asked me how many μL of a particular antibody I used per million cells, and I told him my staining conditions were "per 100 μL" not "per million cells". This triggered a heated debate about antibody amount - he argued that the...