Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
I'm not going to discuss the merits of transforming your log-scaled fluorescence flow cytometry data; I'll leave that to the professionals. What I will try to elucidate here is how I tweak the transformation using FlowJo (Mac version 9.3, sorry Windows people)....
For anyone doing sorting applications, I'd like to clear up a common misconception. Just for the record, cell sorters are not sterile. The instrumentation is not housed in a biosafety cabinet or hood, and therefore it is not possible to sort in a STERILE...