Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
I've been looking for sterility-designed cell sorter for a while and have organized a Tyto demo for our users in 2018 and 2019. I'd like to share what I've learned so far. Let me get some stuff out of the way before we start: 1 – I’m not thinking of the Tyto as a...
Arya Stark from Game of Thrones recites a list of the names of the people she plans to murder at some point or another. I, for one, think that behavior to be odd, somewhat rude and unhealthy. In my mind, I recite a list of the features I'm looking for on cell...
I wouldn't be surprised if the subject of titrating antibodies is one of the least favorite topics among users. We all know someone who skips titrations and just uses the most convenient antibody amount. But as panels get larger, antibody titration becomes much...
When working with patients, samples are rarely collected from all of the patients on the same day. That leaves two options for running samples: cells can be collected and run on the same day over the course of the study or cells can be cryopreserved and run at one...
Last week, the university conducted a vibration study by dropping 5000lb (2268kg) weights from 15ft (4.6m) in four locations near the CAT Facility Lab. In biology terms, that's like dropping a rhinoceros from the height of a giraffe. Why? No idea. But when life...
The voltages on a flow cytometer are one of the more challenging settings to adjust properly. If voltages are too high or too low, the data looks terrible and is potentially unusable. Once voltages have been set and data have been collected there is no way to go...