The voltages on a flow cytometer are one of the more challenging settings to adjust properly. If voltages are too high or too low, the data looks terrible and is potentially unusable. Once voltages have been set and data have been collected there is no way to go back...
We just came back from the Great Lakes International Imaging and Flow Cytometry Association held in Troy, Michigan. While it remains a hidden gem for the greater research community, it is an inescapable meeting for core facility people in the field of flow cytometry...
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