Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
Updated March 25th, 2020 to include more information from Cytek and OMIQ. The COVID-19 pandemic has made a significant impact on our daily lives here in Chicago. Although our facility remains open for now (see facility current status here), we are keeping staff to...
Laura Johnston | Mar 19, 2020 | 0 Comments
Back in 2018, our Cytek Aurora was installed and Cytek came to campus for two days to train us on the instrument. It was all very exciting! A short time passed before researchers really started using the instrument and I was asked to design a panel. From what I...
Laura Johnston | Mar 4, 2020 | 3 Comments
When I was a graduate student, my PI asked me how many μL of a particular antibody I used per million cells, and I told him my staining conditions were "per 100 μL" not "per million cells". This triggered a heated debate about antibody amount - he argued that the...
Laura Johnston | Jan 16, 2020 | 9 Comments
I wouldn't be surprised if the subject of titrating antibodies is one of the least favorite topics among users. We all know someone who skips titrations and just uses the most convenient antibody amount. But as panels get larger, antibody titration becomes much...
Laura Johnston | Nov 15, 2019 | 7 Comments
When working with patients, samples are rarely collected from all of the patients on the same day. That leaves two options for running samples: cells can be collected and run on the same day over the course of the study or cells can be cryopreserved and run at one...
Laura Johnston | Nov 11, 2019 | 7 Comments
The voltages on a flow cytometer are one of the more challenging settings to adjust properly. If voltages are too high or too low, the data looks terrible and is potentially unusable. Once voltages have been set and data have been collected there is no way to go...
Laura Johnston | Oct 18, 2019 | 8 Comments
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