Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
I've been thinking about brightness in flow cytometry lately. It's a simple enough concept, but it's one of these things where you start thinking about it and you may quickly get lost in the numerous moving parts. One could argue that what people really care about...
David Leclerc | May 27, 2021 | 0 Comments
PC issues can be a real showstopper in a flow core, and a humbling one at that. Even for those who spend much of their workweek maintaining and repairing complicated – and expensive! – flow cytometers, fixing an unresponsive keyboard or Blue Screen of Death can be...
Bert Ladd | Mar 3, 2021 | 0 Comments
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
Laura Johnston | Dec 3, 2020 | 8 Comments
In the first post of the "bad data" series, I mostly discussed how to identify problems with cytometer settings and compensation by looking at the data plots. (Read that post here in case you missed it!) In this post I’d like to expand on that by going over common...
Laura Johnston | Nov 19, 2020 | 3 Comments
The manner in which manuscripts acknowledge the work done in Shared Resource Laboratories (SRLs) is inconsistent and an ongoing topic of discussion. These acknowledgements are critical to the survival of SRLs as they are used to satisfy the requirements of federal...
David Leclerc | Nov 9, 2020 | 0 Comments
The Cytek Aurora is not the most polite instrument I know. If you fail a Quality Control test (QC) when you first turn on the instrument, it will spit out a giant FAILED error message in big red letters. For the untrained users, it can be a bit much. I can tell you...
David Leclerc | Oct 8, 2020 | 6 Comments
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