Immunofluorescence is a rapid way to detect antigens in tissue, similar to immunohistochemistry. It is routinely used for renal biopsies, cardiac transplant biopsies, and occasional oral biopsies.

Specimens for IF usually are suspended in Michele’s (or Zeus) fixative.

If you encounter a specimen in this fixative or a specimen labelled for immunofluorescence, consult the front desk. Formalin fixation precludes IF.