Snap Freezing

How to Snap Freeze Tissue for Diagnostics:

  • Take sections of desired tissue (approximately 5 mm cubed). (A good amount of tissue for Neuroblastomas is 2 pieces, 1 gram each).
  • Wrap tissue in foil.
  • Label a megacassette, and place foil-wrapped tissue inside.
  • Submerge in LN2 for 5 minutes.
  • Then, remove cassette using forceps and place in diagnostic box in the -80 freezer.
  • Log specimens into the frozen tissue log book on top of freezer.
  • Write note on triage form.  This information should be included in the gross description.
  • Notify a PA that there is a specimen in the freezer.
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