How to Snap Freeze Tissue for Diagnostics:

• Take sections of desired tissue (approximately 5 mm cubed). (A good amount of tissue for Neuroblastomas is 2 pieces, 1 gram each).
• Wrap tissue in foil.
• Label a megacassette, and place foil-wrapped tissue inside.
• Submerge in LN2 for 5 minutes.
• Then, remove cassette using forceps and place in diagnostic box in the -80 freezer.
• Log specimens into the frozen tissue log book on top of freezer.
• Write note on triage form.  This information should be included in the gross description.
• Notify a PA that there is a specimen in the freezer.